LABORATORY METHODS FOR HERPES VIRUS DIAGNOSIS
Keywords:
Herpes simplex virus, laboratory diagnostics, polymerase chain reaction, virological culture, immunofluorescence, enzyme-linked immunosorbent assay, genotyping, antiviral resistanceAbstract
This article systematically analyzes modern laboratory diagnostic methods for herpes virus infection. Diseases caused by the herpes virus — including labial, genital, ophthalmic herpes, and neuroinfections — are widespread worldwide, making timely and accurate diagnosis highly important. The article comprehensively describes virological methods (virus isolation in cell culture), cytological examination, immunological methods (immunofluorescence, enzyme-linked immunosorbent assay, immunoblot), and molecular genetic methods (polymerase chain reaction, real-time polymerase chain reaction, genotyping, and antiviral resistance genotyping). The sensitivity, specificity, advantages, and limitations of each method are presented based on scientific sources.
References
Abbas, A. K., Lichtman, A. H., & Pillai, S. (2022). Cellular and Molecular Immunology (10th ed.). Elsevier. – bet 289-312
Anderson, N. W., et al. (2014). Light microscopy, culture, molecular, and serologic methods for detection of herpes simplex virus. Journal of Clinical Microbiology, 52(7), 2282-2287. – bet 2284
Badawy, W., & Soltan, A. (2008). Laboratory methods for detection of herpes simplex virus-1 in herpetic keratitis patients. Egyptian Journal of Medical Microbiology, 17(3), 145-152. – bet 148-149
Bailey & Scott's Diagnostic Microbiology (15th ed.). (2020). Elsevier. – bet 567-572
Balda, B. R. (1977). Diagnosis of herpes simplex diseases. Z Hautkr, 52(15), 825-830. – bet 826-827
Bekturdiyev, Sh., & Xodjayeva, D. (2020). Tibbiy mikrobiologiya va immunologiya asoslari. Toshkent: Innovatsiya. – bet 203-205
Evaluation of two rapid methods for the detection of herpes simplex virus antigen in patient specimens. (1985). Annals of Clinical and Laboratory Science, 15(2), 103-109. – bet 105-106
Greninger, A. L., Morrow, R. A., & Jerome, K. R. (2023). Herpes Simplex Viruses and Herpes B Virus. In ClinMicroNow. – bet 12-15
Guidelines for the laboratory diagnosis of genital herpes in eastern European countries. (2010). International Journal of STD & AIDS, 21(8), 533-537. – bet 535
G'ulomov, U., & Salimov, B. (2016). Umumiy mikrobiologiya. Toshkent: O'zMU. – bet 189-192
Iskandarov, I. I. (2020). Immunologiya va immunitet mexanizmlari. Toshkent. – bet 67-68
Madigan, M., Martinko, J., Bender, K., et al. (2018). Brock Biology of Microorganisms (15th ed.). Pearson. – bet 456-458
Muhamedov, I., Eshboyev, E., & Zokirov, N. (2019). Mikrobiologiya, immunologiya va virusologiya. Toshkent: "Moliya". – bet 178-180
Murray, P. R., Rosenthal, K. S., & Pfaller, M. A. (2021). Medical Microbiology (9th ed.). Elsevier. – bet 345-348
Oripov, T., & Yusupova, N. (2018). Tibbiy biologiya va genetikasi. Toshkent: Fan. – bet 234-237
Practical updates in clinical antiviral resistance testing. (2024). Journal of Clinical Microbiology, 62(1), e00221-23. – bet 4-6
Public Health Ontario. (2024). Herpes Simplex (HSV) and Varicella Zoster (VZV) Viruses Molecular testing. PHO Laboratory. Bet 2-5
Raximov, A. (2017). Immunologiya asoslari. Toshkent: O'qituvchi. – bet 101-103
To'ychiyev, O. M., & O'rinboyeva, S. (2018). Mikrobiologiya, virusologiya va immunologiya. Toshkent: TTA. – bet 156-159
Xitoy tibbiyot ensiklopediyasi (腾讯医典). (2023). Herpes simplex virus antibodies. – bet 1-3.
Nath P, Kabir MA, Doust SK, Ray A. Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques. Infect Dis Rep. 2021 Jun 2;13(2):518-539. doi: 10.3390/idr13020049.
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