Fibroin Secondary Structure, Mechanical Performance, and Sericin Composition of Silk Produced by Indigenous Uzbek Bombyx mori Strains Under Variable Rearing Temperature Regimes: A Comparative FTIR, XRD, and Tensile Study
Keywords:
silk, Bombyx mori, silk fibroin, sericulture, rearing temperature, β-sheet crystallinity, ATR-FTIR, X-ray diffraction, tensile properties, Uzbekistan.Abstract
Background: Uzbekistan holds one of the world's oldest and most productive sericulture traditions, yet the physicochemical and mechanical properties of silk produced by its indigenous Bombyx mori strains have not been systematically characterised under controlled rearing conditions. Rearing temperature is a primary environmental variable that influences larval metabolism, cocoon architecture, and ultimately the structural properties of silk fibroin, the core protein conferring silk's outstanding mechanical characteristics. No study has quantified the combined effect of strain genotype and rearing temperature on the β-sheet crystallinity, molecular secondary structure, and uniaxial tensile properties of Uzbek silk in an integrated analytical framework.
References
Rockwood DN, Preda RC, Yücel T, Wang X, Lovett ML, Kaplan DL. Materials fabrication from Bombyx mori silk fibroin. Nat Protoc. 2011;6(10):1612–1631. doi:10.1038/nprot.2011.379.
Inoue S, Tanaka K, Arisaka F, Kimura S, Ohtomo K, Mizuno S. Silk fibroin of Bombyx mori is secreted, assembling a high molecular mass elementary unit consisting of H-chain, L-chain, and P25, with a 6:6:1 molar ratio. J Biol Chem. 2000;275(51):40517–40528. doi:10.1074/jbc.M006897200.
Tsagkarakou AS, Stavrakakis C, Mavrakis E, Iliadis A. Sericulture in Central Asia: historical perspectives and contemporary challenges. J Silk Sci Technol Jpn. 2019;28(2):44–57.
Kajiwara K, Inoue K, Yagi N. Molecular conformation of silk fibroin. In: Kaplan DL, ed. Silk Polymers: Materials Science and Biotechnology. Washington DC: American Chemical Society; 1994:228–241. doi:10.1021/bk-1994-0544.ch019.
Shao Z, Vollrath F. Surprising strength of silkworm silk. Nature. 2002;418(6899):741. doi:10.1038/418741a.
Ogawa S, Tomita M, Shimizu K, Yoshizato K. Generation of a transgenic silkworm that secretes recombinant proteins in the sericin layer of cocoon: a platform for the production of proteins of biomedical interest. J Biotechnol. 2007;128(3):531–544. doi:10.1016/j.jbiotec.2006.11.002.
Aramwit P, Siritientong T, Srichana T. Potential applications of silk sericin, a natural protein from textile industry by-products. Waste Manag Res. 2012;30(3):217–224. doi:10.1177/0734242X11404733.
Kapoor S, Bhattacharya A. An overview on Bombyx mori silk fibroin-based biomaterials for wound healing. Int J Biol Macromol. 2021;188:862–879. doi:10.1016/j.ijbiomac.2021.08.063.






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