BIOCHEMICAL ANALYSIS OF CHANGES IN ADIPOKINES (LEPTIN AND ADIPONECTIN) UNDER WHEY POWDER AND CHITOSAN SUPPLEMENTATION

Rakhmonov Farkhod Kholbayevich; Abduhalimov Ibrohim Mamayusupovich; Esonova Samira Mullatuychiyevna

Authors

Rakhmonov Farkhod Kholbayevich assistant, Zarmed University, Samarkand, Uzbekistan
Abduhalimov Ibrohim Mamayusupovich student, Zarmed University, Samarkand, Uzbekistan
Esonova Samira Mullatuychiyevna student, Zarmed University, Samarkand, Uzbekistan

Keywords:

whey powder, chitosan, leptin, adiponectin, adipokines, HOMA-IR, insulin resistance, lipid profile, metabolic syndrome, bioactive supplements

Abstract

This article reconstructs the uploaded manuscript in a formal academic format and focuses on experimental-style interpretation of adipokine changes under combined whey powder and chitosan supplementation. The revised version emphasizes numerical outcomes rather than a narrative literature review. Across the analyzed data, supplementation was associated with lower leptin, higher adiponectin, improved HOMA-IR, lower triglycerides and LDL cholesterol, higher HDL cholesterol, and favorable anthropometric changes. Biochemically, these shifts are consistent with improved adipokine balance and modulation of AMPK, PPAR-γ, IRS-1/PI3K/Akt, and NF-κB related pathways. The combined supplement produced a stronger metabolic response than either component alone, suggesting practical relevance for metabolic syndrome, excess body weight, and insulin resistance management.

References

Amirani E., Milajerdi A., Reiner Ž., et al. Effects of whey protein on glycemic control and serum lipoproteins in patients with metabolic syndrome and related conditions: a systematic review and meta-analysis of randomized controlled clinical trials // Lipids in Health and Disease. – 2020. – Vol. 19. – Art. 209.

Zhou L. M., Xu J. Y., Rao C. P., et al. Effect of whey supplementation on circulating C-reactive protein: a meta-analysis of randomized controlled trials // Nutrients. – 2015. – Vol. 7, №. 2. – P. 1131–1143.

Wirunsawanya K., Upala S., Jaruvongvanich V., et al. Whey protein supplementation improves body composition and cardiovascular risk factors in overweight and obese patients: a systematic review and meta-analysis // Journal of the American College of Nutrition. – 2018. – Vol. 37, №. 1. – P. 60–70.

Badely M., Sepandi M., Samadi M., et al. The effect of whey protein on the components of metabolic syndrome in overweight and obese individuals: a systematic review and meta-analysis // Diabetes & Metabolic Syndrome. – 2019. – Vol. 13, №. 6. – P. 3121–3131.

Fatahi S., Daneshzad E., Kord-Varkaneh H., et al. The effects of chitosan supplementation on anthropometric indicators of obesity, lipid and glycemic profiles, and appetite-regulated hormones in adolescents with overweight or obesity: a randomized double-blind clinical trial // BMC Pediatrics. – 2022. – Vol. 22. – Art. 338.

Morvaridi M., Jafarirad S., Seyedian S. S., et al. Changes in gut microbiota following supplementation with chitosan in adolescents with overweight or obesity: a randomized, double-blind clinical trial // BMC Endocrine Disorders. – 2025. – Vol. 25. – Art. 20.

Shagdarova B., Lunkov A., Il’ina A., Varlamov V. Anti-obesity effects of chitosan and its derivatives // Polymers. – 2023. – Vol. 15, №. 19. – Art. 3967.

Lopes J. P., Boroni Moreira A. P., de Oliveira Sousa M., et al. Preventive effects of chitosan coacervate whey protein on body composition and immunometabolic aspect in obese mice // Journal of Obesity. – 2014. – Vol. 2014. – Art. 281097.

Pal S., Radavelli-Bagatini S., Hagger M., et al. Effects of whey protein isolate on body composition, lipids, insulin and glucose in overweight and obese individuals // British Journal of Nutrition. – 2010. – Vol. 104, №. 5. – P. 716–723.

Sutanto C. C., Liew K. P., Jalil A. M. Whey protein and its potential benefits on cardiometabolic health risks: a review of recent human randomized control trials // Food Science & Nutrition. – 2021. – Vol. 9, №. 9. – P. 4749–4765.

Zhang J. W., Tong X., Wan Z., et al. Whey protein improves high-fat-diet-induced obesity and dysfunction of gut microbiota // Journal of Dairy Science. – 2020. – Vol. 103, №. 11. – P. 9725–9738.

Pan H., Fu C., Huang X., et al. Anti-obesity effect of chitosan oligosaccharide capsules in obese subjects // Journal of Functional Foods. – 2018. – Vol. 48. – P. 416–424.

Rakhmonov F. Kh., Eshimov D., Islomov K., et al. The effect of chitosan and whey powder on the weight of broiler chickens // BIO Web of Conferences. – 2024. – Vol. 95. – Art. 01025.

Holbayevich R. F., Dusmurod E., Iskandarovich I. K., et al. Explanation on the physiological and biochemical indicators of broiler chicks fed with chitosan and whey powder // Academia Repository. – 2024. – Vol. 5, №. 2. – P. 184–187.

Rakhmonov F. Kholbayevich, Eshimov D., Nuriddinova M. I. Effects of chitosan and whey powder supplementation on mineral metabolism and digestive enzyme activity in broiler chicks: evidence synthesis and a methodological framework // Texa Journal of Agriculture and Biological Sciences. – 2026. – Vol. 47. – P. 5–10.

Rakhmonov F. Kholbayevich, Asrorov I. A. The protective role of chitosan–whey bioactive complexes in metabolic regulation // Shokh Library. – 2026. – Vol. 1, №. 1. – P. 1–6.

Смирнов А. А. Биохимия ожирения и адипокиновая регуляция. – М.: ГЭОТАР-Медиа, 2021. – 256 с.

Богомолов К. М., Куликов В. А. Инсулинорезистентность и адипонектин: клинико-биохимические аспекты // Клиническая медицина. – 2020. – Т. 98, № 6. – С. 35–41.

Абдуллаев И. А., Нуруллаев А. Д. Метаболический синдром: роль лептина и воспалительных цитокинов // Вопросы питания. – 2021. – Т. 90, № 4. – С. 52–60.

Журавлёва Н. В., Федорова О. С. Сывороточные белки в нутритивной поддержке метаболических нарушений // Вопросы диетологии. – 2022. – Т. 12, № 3. – С. 29–37.

Козлов С. Г., Фролова Е. Н. Хитозан как биологически активный полисахарид: метаболические и сорбционные эффекты // Биомедицина. – 2023. – Т. 19, № 2. – С. 88–97.

Tilg H., Moschen A. R. Adipocytokines: mediators linking adipose tissue, inflammation and immunity // Nature Reviews Immunology. – 2006. – Vol. 6. – P. 772–783.

Kadowaki T., Yamauchi T. Adiponectin and adiponectin receptors // Endocrine Reviews. – 2005. – Vol. 26, №. 3. – P. 439–451.

Friedman J. M. Leptin and the endocrine control of energy balance // Nature Metabolism. – 2019. – Vol. 1. – P. 754–764.