THE PROTECTIVE ROLE OF CHITOSAN–WHEY BIOACTIVE ADDITIVES IN MAINTAINING CELL MEMBRANE STABILITY AND REDUCING LIPID PEROXIDATION

Rakhmonov Farkhod Kholbayevich, Asrorov Ixtiyorjon Alisher ugli

Authors

Rakhmonov Farkhod Kholbayevich, Asrorov Ixtiyorjon Alisher ugli Zarmed University, Samarkand, Uzbekistan

Keywords:

chitosan, whey powder, lipid peroxidation, membrane stability, oxidative stress, MDA, antioxidant synergy, bioactive compound.

Abstract

Oxidative stress is one of the most critical biochemical factors impairing cellular integrity through the initiation of lipid peroxidation (LPO). Chitosan and whey powder, as natural bioactive compounds, exhibit strong antioxidant and membrane-stabilizing properties. This study provides an in-depth evaluation of the synergistic role of chitosan–whey bioactive additives in reducing malondialdehyde (MDA) formation, restoring membrane phospholipids, and enhancing antioxidant enzyme activity. Mechanistic analysis demonstrates that chitosan effectively scavenges free radicals and interacts electrostatically with membrane lipids, while whey proteins and peptides support membrane regeneration and strengthen endogenous antioxidant defense systems. Evidence from experimental models indicates that combined supplementation significantly reduces oxidative damage compared to the individual components. These findings establish chitosan–whey complexes as promising membrane-protective agents for biomedical, veterinary, and nutritional applications.

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