EVALUATING MOLECULAR IDENTIFICATION TECHNIQUES FOR PATHOGENIC FUNGI ON PISTACHIOS: A COMPREHENSIVE LITERATURE REVIEW ON PRIMER MARKERS AND METHODOLOGICAL GUIDELINES

Authors

Odina Nazarova Jumadullaevna Research institute of Forestry, Uzbekistan

Keywords:

Pistachio, phytopathogenic fungi, molecular identification, PCR, primer markers, PCR-RFLP, microsatellites, phylogenetics, sustainable production.

Abstract

This article reviews molecular identification methods applied to pathogenic fungi affecting pistachio cultivation. In particular, the use of PCR and primer markers is analyzed, emphasizing their advantages in terms of accuracy, rapidity, and efficiency compared to traditional morphological methods. The importance of selecting appropriate primers for differentiating fungal species is highlighted, as well as the application of microsatellite markers, PCR-RFLP, and phylogenetic analyses. The article demonstrates the opportunities for integrating modern molecular diagnostics into agricultural practice, with the ultimate goal of safeguarding pistachio yield and quality.

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Published

2025-10-31

How to Cite

Odina Nazarova Jumadullaevna. (2025). EVALUATING MOLECULAR IDENTIFICATION TECHNIQUES FOR PATHOGENIC FUNGI ON PISTACHIOS: A COMPREHENSIVE LITERATURE REVIEW ON PRIMER MARKERS AND METHODOLOGICAL GUIDELINES. Ethiopian International Journal of Multidisciplinary Research, 12(10), 822–827. Retrieved from https://eijmr.org/index.php/eijmr/article/view/3823